Bonn 2012

Link

http://2012.igem.org/Team:Bonn/Project#LOV_Kills

Class

# Format: # Write how you would classify them.

Toxins

Name	Number	Comment
ccdB		attacks gyrase (a topoisomerase II) which results in the blockade of polymerases.

Antagonists

Name	Number	Comment
ccdA		inhibits the toxic activity by forming a ccdA:ccdB complex

Topology

Parts

Name	Number	Comment
LOV2 2012	BBa_K820000	contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm).
promoter (lacI regulated)	BBa_R0010	In the absence of LacI protein and CAP protein this part promotes transcription.
RBS	BBa_B0032	Ribosom binding site
T1	BBa_B0010	T1 from e. coli rrnB
TE	BBa_B0012	Transcription terminator for the E.coli RNA polymerase

Link

http://2012.igem.org/Team:Bonn/Project#LOV_Kills

Class

# Format: # Write how you would classify them.

Toxins

Name	Number	Comment
mazF		is a sequence-specific ribonuclease from B. subtilis and E. coli.

Antagonists

Name	Number	Comment
mazE		counterbalance mazF

Topology

Parts

Name	Number	Comment
LOV2 2012	BBa_K820000	contains a photo-activable LOV2 domain which changes its conformation upon exposure to blue light (420-480 nm).
promoter (lacI regulated)	BBa_R0010	In the absence of LacI protein and CAP protein this part promotes transcription.
RBS	BBa_B0032	Ribosom binding site
T1	BBa_B0010	T1 from e. coli rrnB
TE	BBa_B0012	Transcription terminator for the E.coli RNA polymerase
mazF	BBa_K302033	Encodes a stable sequence-specific ribonuclease